Thesis Title

Isolation And Characterization Of Soil Microorganisms Capable Of Utilizing Used Motor Oil For Growth

Author

Robert Sallee

Date of Graduation

Summer 2001

Degree

Master of Science in Biology

Department

Biology

Committee Chair

John Steiert

Subject Categories

Biology

Abstract

Bioremediation research of petroleum products is extensive and ongoing, but there is a general lack of research dealing specifically with used-motor oil. The biodegration of used motor oil using inoculums from pristine soil and used-motor oil contaminated soil was investigated. Six indigenous soil microorganisms that were capable of utilizing used-motor oil as their sole carbon-energy source were isolated from oil agar plates. These microorganisms were identified by either the 16S rRNA gene, or the 18S rRNA gene DNA sequences. Classical methods of identification were performed as a supplementation to the molecular method. The isolates were analyzed for their ability to degrade various hydrocarbons, such as n-alkanes, branched alkanes, cycloalkanes, n-alkenes, aromatics, and polycyclic aromatics. The six isolates were characterized for the optimal growth temperature, pH, and salinity. The genera of the six isolates were Pseudomonas, Xanthomonas, Methylobacterium, Sphingomonas, Aspergillus, and Eupenicillium.

Copyright

© Robert Sallee

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