Date of Graduation

Spring 2011

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Kyoungtae Kim

Keywords

eisosome, Pil1, synaptojanin, amphiphysin, receptor-mediated endocytosis, yeast

Subject Categories

Biology

Abstract

The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 and Las17 persisted much longer in PIL1-deficient cells (pil1Δ). The loss of Pil1 also greatly affected both the scission efficiency and the frequency of formation of endocytic sites carrying Rvs161- and Rvs167-GFP. Furthermore, the mistargeting of the synaptojanins, Sjl1 and Sjl2, to the cytoplasm in pil1Δ cells suggests that Pil1 is required for the proper recruitment of the synaptojanins to endocytic sites. A severe motility defect of Abp1-GFP during its internalization in a codeletant of PIL1 and SJL2 indicates a functional interplay between them in endocytosis. Together, these results establish that Pil1 is involved in the recruitment of endocytic proteins to optimize endocytosis.

Copyright

© Erin Ruth Murphy

Campus Only

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