Thesis Title

An Evaluation of Two Interleukin-1 Assays

Date of Graduation

Summer 1989

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Richard Myers

Subject Categories

Biology

Abstract

Interleukin-1 (IL-1) is produced by macrophages and has been shown to be an important protein involved in the regulation of an immune response. Several methods of detecting IL-1 in the supernatant of macrophage cultures have been developed. The purpose of this project was to evaluate two of these methods. The two assays used were based on the fact that T cells proliferate in the presence of IL-1 and plant lectins. These assays differed with respect to the source of the T cells: nylon wool purified or D10.G4.1 cell line. Methods involving the use of D10.G4.1 cells were founds to be more accurate than methods involving the use of nylon wool purified T cells.

Copyright

© Frank Anthony DiVincenzo

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Dissertation/Thesis

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