Thesis Title

Susceptibility Testing of Pathogenic Fungi With a Radiometric System

Date of Graduation

Fall 1986

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Richard Myers

Subject Categories

Biology

Abstract

A radiomethric method was used to determine the susceptibility of clinical isolates of dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, Sporothrix schenckii) to selected antifungal agents. Antifungal agents evaluated were Amphotericin B, 5-fluorocytosine, Nystatin, Ketoconazole, Miconazole, Terconazole, and Itraconazole. The instrument used to measure amount of radioactive carbon dioxide generated by fungal metabolism of ¹⁴C-glucose in presence of antifungal agents was the Bactex 460. Mycelial cultures were grown on SAB-DEX agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast growth in one-third the time required with conventional susceptibility test methods. This radiometric method has potential for routine laboratory use in evaluating antibiotic susceptibility of fungi

Copyright

© Rhonda Gay Allen

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