Date of Graduation

Fall 2014

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Wenping Qiu

Keywords

Grapevine vein clearing virus, GVCV-VRU, Badnavirus, virus isolate, polymerase chain reaction

Subject Categories

Plant Sciences

Abstract

Grapevine vein clearing virus (GVCV) was the first DNA virus discovered in commercial Vitis vinifera L. varieties. GVCV is classified under the Badnavirus genus, in the Caulimoviridae family. The virus poses an increasing threat to grape and wine industries in the Midwest region of the United States. GVCV causes decline in developmental growth that can eventually lead to the death of the grapevine(s). A new isolate of GVCV was found and sequenced. The new isolate was referred to as GVCV-VRU as was is discovered in wild V. rupestris. Assays of cultivated grapevines were performed by using polymerase chain reaction (PCR) to detect GVCV-VRU. Results showed that GVCV-VRU exist in grape cultivars in commercial vineyards. GVCV-VRU infected grapevines were also grafted onto five different grape cultivars. PCR assays indicated that Chardonel, Vidal Blanc, V. riparia, and Vignoles are infected with GVCV-VRU by graft transmission while GVCV-VRU was not detected in grafted Chambourcin. These results provide important clues on the possible routes of GVCV epidemics.

Copyright

© LeAnn Catherine Hubbert

Campus Only

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