Date of Graduation

Summer 2010

Degree

Master of Science in Chemistry

Department

Chemistry

Committee Chair

Erich Steinle

Keywords

aptamer, gold nanoparticles, dynamic light scattering, lateral flow, bound glass, nanoconjugates, thrombin

Subject Categories

Chemistry

Abstract

This thesis describes rapid, facile, selective, and specific solution and surface-based assays for the detection of proteins. Specifically, thrombin is used in both as a model system in conjunction with aptamer-gold nanoconjugates. The solution-based method utilizes Dynamic Light Scattering Spectroscopy (DLSS) as a method to detect increases in hydrodynamic diameter size of the conjugates. In the surface-based method, lateral flow technology is applied in conjunction with the aptamer-gold nanoconjugates to show both positive and control lines on the novel bound glass test strip. Both of these assays have significant biomedical and chemical applications.

Copyright

© Tiffany Leah Severs

Campus Only

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