Date of Graduation
Master of Science in Chemistry
aptamer, gold nanoparticles, dynamic light scattering, lateral flow, bound glass, nanoconjugates, thrombin
This thesis describes rapid, facile, selective, and specific solution and surface-based assays for the detection of proteins. Specifically, thrombin is used in both as a model system in conjunction with aptamer-gold nanoconjugates. The solution-based method utilizes Dynamic Light Scattering Spectroscopy (DLSS) as a method to detect increases in hydrodynamic diameter size of the conjugates. In the surface-based method, lateral flow technology is applied in conjunction with the aptamer-gold nanoconjugates to show both positive and control lines on the novel bound glass test strip. Both of these assays have significant biomedical and chemical applications.
© Tiffany Leah Severs
Severs, Tiffany Leah, "Synthesis, Modification, Characterization and Bioanalytical Applications of Aptamer-Gold Nanoconjugates" (2010). MSU Graduate Theses. 1884.