Date of Graduation

Spring 2016

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Chin-Feng Hwang

Keywords

Norton, Cynthiana, Chambourcin, Cabernet Sauvignon, microsatellite markers, hybrids, comparative assessment, linkage map, JoinMap

Subject Categories

Plant Sciences

Abstract

The first part of this study utilized microsatellites to comparatively assess the cultivars Norton and Cynthiana. Although isozyme and simple sequence repeat (SSR) marker analyses in 1993 and 2009 provided preliminary evidence that Norton and Cynthiana grapes are genetically identical, only five banding patterns and four microsatellite loci were reported. Microsatellites (n=185) spanning 19 linkage groups were used to compare the cultivars for a genome-wide analysis. Capillary electrophoresis results revealed Norton and Cynthiana to be identical at 98.6% of alleles. In the second part of this study, an interspecific hybrid population was generated by crossing V. interspecific hybrid 'Chambourcin' and V. vinifera 'Cabernet Sauvignon'. The ultimate goal of performing this cross is to create a cultivar with the cold hardiness of 'Chambourcin' combined with the superior wine quality of V. vinifera 'Cabernet Sauvignon'. Cross-population (CP) maps were generated using the statistical software JoinMap 4.1 by genotyping 90 F1 progenies using microsatellites. Map sizes ranged from 999.3 cM to 1821.9 cM and contained a maximum of 276 SSR markers.

Copyright

© Mia Elizabeth Mann

Open Access

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