Author

Mary M. Dean

Date of Graduation

Spring 2008

Degree

Master of Natural and Applied Science in Agriculture

Department

College of Agriculture

Committee Chair

Gary Webb

Keywords

stallion, spermatozoa, centrifuge, percent recovery, post-storage

Subject Categories

Agriculture

Abstract

Two experiments were conducted in order to compare the effects of centrifugation on post-storage motility, percent intact acrosomes, detached heads and recovery of spermatozoa. In experiment 1, one aliquot was diluted with extender and placed in a 50 ml tube and centrifuged for 700 x g for 15 min. Additional aliquots were diluted 1:1, placed in 15 ml tubes and centrifuged at 400 x g for 7 min. In experiment 2, two aliquots were diluted and placed in 50 ml tubes and centrifuged for either 700 x g for 15 min or 600 x g for 12 min. The procedure for centrifugation at 400 x g was repeated. Following centrifugation, aliquots from each treatment were stored at 5o C or frozen in liquid nitrogen. In experiment 1, cold stored aliquots centrifuged for 400 x g yielded higher total and progressive motility, average path and straight line velocity and percent recovery for cold stored treatments (P≤ 0.05). In experiment 2, cold storage total motility was significantly higher (P≤ 0.01) for aliquots centrifuged at 400 x g in 15 ml tubes as compared to those centrifuged in 50 ml tubes at higher speeds and longer times. Post-thaw motility was not different between centrifugation treatments for either experiment. Following centrifugation, the percent recovery was higher (P≤ 0.01) for aliquots centrifuged in the 15 ml vs. 50 ml tubes for both experiments. However, spermatozoal integrity was not different between treatments for both experiments.

Copyright

© Mary M. Dean

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