Date of Graduation

Fall 2016

Degree

Master of Science in Chemistry

Department

Chemistry

Committee Chair

Gary Meints

Keywords

oxo-A, NMR, NOESY, DNA, base-excision repair, Gaussian, density functional theory, 3D structure, Dickerson dodecamer

Subject Categories

Chemistry

Abstract

Since the characterization of the oligomer d(CGCGAATTCGCG) has been published by Dickerson et al., computational studies have been carried out to produce an accurate 3D model. These models are important for visualizing how certain DNA repair enzymes, such as the glycosylases, recognize sites of damage by signatures of local 3D distortion. Using 1H NOESY-generated internuclear distances to replicate the model of this oligomer and a derivative with an 8-oxo-dA5 lesion, we propose characteristics of helical distortion that DNA glycosylases might use for identifying this form of damage. In addition, this method of comparison can be used to study the repair signatures of other known DNA lesions.

Copyright

© Christopher Miles Reynolds

Open Access

Included in

Chemistry Commons

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