Thesis Title

Evidence of Bacteriocinogeny in Yersinia Ruckeri

Date of Graduation

Summer 1988

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Russell Barnekow

Subject Categories

Biology

Abstract

Twelve isolates of Yersinia ruckeri were examined for bacteriocinogeny. Seven isolates produced an antagonistic substance that inhibited five other Y. ruckeri isolates, as demonstrated by the modified double layer method. Producer isolates were immune to the antagonistic substance. Extracts prepared by freezing and thawing of the producer isolates exhibited antagonistic activity demonstrated by a thin layer technique. The substance is a heat stable, chloroform resistant, trypsin sensitive protein with an estimated molecular weight of 60,000 to 65,000. Plasmid control is suggested by the presence of a 72 MDa plasmid present in producer isolates. Susceptibility or resistance of isolates is not related to serovar group, clone assignment, or sorbitol biotype. It is proposed that this new bacteriocin be named ruckerin.

Copyright

© Elmer W Otteson

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Dissertation/Thesis

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