Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus
Date of Graduation
Master of Science in Biology
Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.
© Annette Renee Stewart
Stewart, Annette Renee, "Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus" (1986). MSU Graduate Theses. 65.