Thesis Title

Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus

Date of Graduation

Summer 1986

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Christopher Field

Subject Categories

Biology

Abstract

Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.

Copyright

© Annette Renee Stewart

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