Thesis Title

Steroid Stimulated Protein Synthesis in Fish Cell Cultures

Date of Graduation

Spring 1986

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Christopher Field

Subject Categories

Biology

Abstract

Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.

Copyright

© Carla B Green

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Dissertation/Thesis

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