Thesis Title

Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna

Date of Graduation

Summer 1986

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Christopher Field

Subject Categories

Biology

Abstract

Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed.

Copyright

© Mark Henry Entrup

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Dissertation/Thesis

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