Selective inhibition of Alu retrotransposition by APOBEC3G

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LINE-1, retrotransposon, APOBEC, transposable element


The non-LTR retrotransposon LINE-1 (L1) comprises ∼ 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an ORF1p-independent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G.

Recommended Citation

Hulme, Amy E., Hal P. Bogerd, Bryan R. Cullen, and John V. Moran. "Selective inhibition of Alu retrotransposition by APOBEC3G." Gene 390, no. 1-2 (2007): 199-205.

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