Abstract

Understanding how root systems modulate shoot system phenotypes is a fundamental question in plant biology and will be useful in developing resilient agricultural crops. Grafting is a common horticultural practice that joins the roots (rootstock) of one plant to the shoot (scion) of another, providing an excellent method for investigating how these two organ systems affect each other. In this study, we used the French-American hybrid grapevine ‘Chambourcin’ (Vitis L.) as a model to explore the rootstock–scion relationship. We examined leaf shape, ion concentrations, and gene expression in ‘Chambourcin’ grown ungrafted as well as grafted to three different rootstocks (‘SO4’, ‘1103P’ and ‘3309C’) across 2 years and three different irrigation treatments. We found that a significant amount of the variation in leaf shape could be explained by the interaction between rootstock and irrigation. For ion concentrations, the primary source of variation identified was the position of a leaf in a shoot, although rootstock and rootstock by irrigation interaction also explained a significant amount of variation for most ions. Lastly, we found rootstock-specific patterns of gene expression in grafted plants when compared to ungrafted vines. Thus, our work reveals the subtle and complex effect of grafting on ‘Chambourcin’ leaf morphology, ionomics, and gene expression.

Department(s)

Biology

Document Type

Article

Additional Information

Supplementary Information accompanies this paper at https://doi.org/10.1038/s41438-019-0146-2.

This work was supported by Missouri Grape and Wine Institute, National Science Foundation Plant Genome Research Program 1546869, and Saint Louis University. This work was partially supported by appropriated funds to USDAARS-GGRU for project 8060-21220-006-00D. This project was also supported by the USDA National Institute of Food and Agriculture, and by Michigan State University AgBioResearch.

DOI

https://doi.org/10.1038/s41438-019-0146-2

Rights Information

© The Authors 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Publication Date

5-1-2019

Journal Title

Horticulture Research

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