Pyruvate affects inflammatory responses of macrophages during influenza A virus infection
Abstract
Pyruvate is the end product of glycolysis and transported into the mitochondria for use in the tricarboxylic acid (TCA) cycle. It is also a common additive in cell culture media. We discovered that inclusion of sodium pyruvate in culture media during infection of mouse bone marrow derived macrophages with influenza A virus impaired cytokine production (IL-6, IL-1β, and TNF-α). Sodium pyruvate did not inhibit viral RNA replication. Instead, the addition of sodium pyruvate alters cellular metabolism and diminished mitochondrial reactive oxygen species (ROS) production and lowered immune signaling. Overall, sodium pyruvate affects the immune response produced by macrophages but does not inhibit virus replication.
Department(s)
Biology
Document Type
Article
DOI
https://doi.org/10.1016/j.virusres.2020.198088
Keywords
Antioxidant, Inflammasome, Inflammation, Influenza A virus, Pyruvate
Publication Date
9-1-2020
Recommended Citation
Abusalamah, Hazar, Jessica M. Reel, and Christopher R. Lupfer. "Pyruvate affects inflammatory responses of macrophages during influenza A virus infection." Virus research 286 (2020): 198088.
Journal Title
Virus Research
