The dynamic impact of CpG methylation in DNA
Solid-state deuterium NMR is used to investigate perturbations of the local, internal dynamics in the EcoRI restriction binding site, -GAATTC- induced by cytidine methylation. Methylation of the cytidine base in this sequence is known to suppress hydrolysis by the EcoRI restriction enzyme. Previous solid-state deuterium NMR studies have detected large amplitude motions of the phosphate-sugar backbone at the AT-CG junction of the unmethylated DNA sequence. This study shows that methylation of the cytidine base in a CpG dinucleotide reduces the amplitudes of motions of the phosphate-sugar backbone. These observations suggest a direct link between suppression of the amplitudes of localized, internal motions of the sugar- phosphate backbone of the DNA and inhibition of restriction enzyme cleavage.
Geahigan, Karen B., Gary A. Meints, Mary E. Hatcher, John Orban, and Gary P. Drobny. "The dynamic impact of CpG methylation in DNA." Biochemistry 39, no. 16 (2000): 4939-4946.