Title

Facile Synthesis of a Fluorescent Deoxycytidine Analogue Suitable for Probing the RecA Nucleoprotein Filament

Abstract

We report the synthesis of the fluorescent 2‘-deoxycytidine analogue 5-methylpyrimidin-2-one nucleoside, its incorporation at three specified sites in a single 60-nucleotide DNA molecule, and the use of its total and polarized intrinsic fluorescence to characterize RecA−DNA complexes.

Document Type

Article

DOI

https://doi.org/10.1021/ol0167863

Keywords

filaments, nucleic acids, genetics, monomers, fluorescence

Publication Date

2001

Recommended Citation

Singleton, Scott F., Feng Shan, Matthew W. Kanan, Catherine M. McIntosh, Chad J. Stearman, Jeremiah S. Helm, and Kristofor J. Webb. "Facile synthesis of a fluorescent deoxycytidine analogue suitable for probing the RecA nucleoprotein filament." Organic letters 3, no. 24 (2001): 3919-3922.

Journal Title

Organic letters

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