Title

Profiling the yeast communities of wine fermentation using terminal restriction fragment length polymorphism analysis

Document Type

Article

Publication Date

2011

Abstract

Terminal restriction fragment length polymorphism (TRFLP), used most often to describe bacterial communities, presents a high-throughput, low-cost solution for analyzing mixed yeast communities in wine and other fermentations. In this study, a TRFLP approach was developed for the identification and discrimination of yeasts and used to construct a TRFLP database comprising 121 strains of yeast (representing 24 genera and 72 species) associated with wine and food fermentations. This database exhibits sensitive discrimination among species and robust intra-specific conservation of TRFLP profiles, enabling reliable characterization of mixed yeast communities. The yeast ecology of a sweet, botrytized wine fermentation from two separate vintages was analyzed using this database, demonstrating the utility of this method for fast-paced, qualitative detection and identification of differences in yeast community structures over time in a complex, diverse fermentation system.

Recommended Citation

Bokulich, Nicholas, Chin-Feng Hwang, Shuwen Liu, Kyria Boundy-Mills, and David Mills. Profiling the yeast communities of wine fermentation using terminal restriction fragment length polymorphism analysis." American journal of enology and viticulture (2011): ajev-2011."

DOI for the article

http://dx.doi.org/10.5344/ajev.2011.11077

Department

College of Agriculture

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