An Evaluation of Two Interleukin-1 Assays
Date of Graduation
Master of Science in Biology
Interleukin-1 (IL-1) is produced by macrophages and has been shown to be an important protein involved in the regulation of an immune response. Several methods of detecting IL-1 in the supernatant of macrophage cultures have been developed. The purpose of this project was to evaluate two of these methods. The two assays used were based on the fact that T cells proliferate in the presence of IL-1 and plant lectins. These assays differed with respect to the source of the T cells: nylon wool purified or D10.G4.1 cell line. Methods involving the use of D10.G4.1 cells were founds to be more accurate than methods involving the use of nylon wool purified T cells.
© Frank Anthony DiVincenzo
DiVincenzo, Frank Anthony, "An Evaluation of Two Interleukin-1 Assays" (1989). MSU Graduate Theses. 143.