Transgenic Plants, Current State, and Influence on the Environment
Date of Graduation
Master of Science in Biology
The techniques of plant transformation are discussed. They are bacterial-mediated, electroporation transformation, biolistic (particle bombardment), and microbeam laser. By using different strategies, foreign gene(s) from various organisms can be inserted into plant genome and expressed. Bacteria-mediated transformation uses Agrobacterium as a vector to insert genes of interest. The other three directly deliver the plasmid into the plant. While Bacteria-mediated method is relatively simple, the other three can transform both monocot and dicot plant, and use various plant tissues as target cells. Public concerns about the impact that transgenic plant might have on the environment have partly slowed down the large-scale use of transgenic plants. These concerns are discussed. Some suggestions regarding the releasing of the transgenic plant under field conditions are also discussed.
© Xian Xie
Xie, Xian, "Transgenic Plants, Current State, and Influence on the Environment" (1998). MSU Graduate Theses. 182.