Thesis Title

Expression of the Ta-Iaam Gene of Agrobacterium Vitis in Petunia Hybrida

Author

Lilly Boppuri

Date of Graduation

Fall 1998

Degree

Master of Science in Plant Science

Department

Biology

Committee Chair

Laszlo Kovacs

Subject Categories

Plant Sciences

Abstract

I cloned the TA-iaaM gene, an auxin biosynthetic oncogene from A. vitis strain Tm-4, behind the 35S promoter of the cauliflower mosaic virus in an Agrobacterium binary plasmid. The presence of the gene in the resulting plasmid was confirmed with restriction digestion and PCR analyses. The construct was introduced into an Agrobacterium transformation vector, and transferred into Petunia hybrida cells by the leaf disc transformation method. The resulting transgenic plants exhibited morphological abnormalitites, including lancet shaped, downward curling leaves, and strong apical dominance, indicating the expression of the transgene, and the accumulation of excessive amounts of indole-3-acetic acid. Transgenic plants of normal morphological features have also been obtained. These latter plants may have an inactivated or silenced transgene.

Copyright

© Lilly Boppuri

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