Date of Graduation

Summer 2016


Master of Science in Biology



Committee Chair

Kyoungtae Kim


Vps1, ESCRT, Protein Sorting, MVB, Yeast-Two Hybrid Screen

Subject Categories



Yeast dynamin, Vps1, is a dynamic protein implicated in multiple trafficking pathways and at several cellular locations including the Golgi, endosome, and vacuole. Previous studies have found that Vps1 genetically and physically interacts with several ESCRT-II (Vps22, Vps36) and ESCRT-III (Vps2, Vps24) components. In light of these findings, little is known about how Vps1 functions with the ESCRT system. In my study, I have mapped the interaction domains between Vps1 and Vps22/Vps36 as well as explored the potential roles of these proteins during the targeting of vacuolar hydrolase, Cps1. My results support the idea of Vps1 working at the endosomal membrane and interacting with ESCRT-II towards the N-terminal end of its Vps22/Vp36 lobe. Additionally, a yeast two-hybrid library screen was performed in search of Vps1 binding partners. Here, I present seventeen novel binding partners. Based on these results, I have proposed a potential role for Vps1 in regulating chitin synthases during yeast cellular budding.


© Bryan Thien Banh

Open Access

Included in

Biology Commons