Date of Graduation

Spring 2018

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Wenping Qiu

Keywords

stilbene synthase, Botrytis cinerea, GVCV, genomic feature, virus

Subject Categories

Biotechnology | Plant Pathology

Abstract

Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three times. The results showed that the STS-overexpressing Arabidopsis did not show resistance to B. cinerea, indicating that single STS gene might not be fully functional in defending against B. cinerea. In a second project, two new Grapevine vein clearing virus (GVCV) isolates were sequenced and analyzed. The two GVCV isolates have 99.8% identical nucleotide sequence, thus were considered as same strain although they were isolated from two plants in different genera of the Vitaceae family. The conserved domains, secondary structure of leader RNA, and phylogenetic relationship were predicted among seven isolates. The results provide new evidence that GVCV spreads across plants in different genera. Thus, it is urgent to take measures for preventing virus spread.

Copyright

© Li Su

Open Access

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