Date of Graduation

Summer 2018

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Chin-Feng Hwang

Keywords

Jaeger 70, Vignoles, molecular breeding, SSR, microsatellite, Hermann Jaeger, hybrids, vitis, Munson

Subject Categories

Agriculture | Genetics and Genomics | Molecular Genetics | Viticulture and Oenology

Abstract

Jaeger 70 cultivar is an interspecific hybrid of Vitis linececumii x Vitis rupestris, with an interesting past. It was created by Hermann Jaeger of Neosho, MO, who is most notable for saving the French wine industry by developing phylloxera resistant rootstocks in the mid-1800s. Traditionally the Jaeger 70 variety has been grafted to French wine grape cultivars to impart disease resistance and cold hardiness while retaining desirable wine characteristics. One interesting characteristic of Jaeger 70 is that it is a female-only plant, allowing for many interesting areas of study. Vitis interspecific hybrid Vignoles, is a cold hardy cultivar of largely unknown parentage which produces high quality sweet fruity wines. Vignoles, however, produces compact clusters that are susceptible to bunch rot. In comparison, Jaeger 70 produces a moderately compact berry cluster which is highly resistant to bunch rot. The purpose of this study was to screen 508 progenies for hybrid confirmation of an F1 interspecific hybridization of Jaeger 70 x Vignoles using SSR (Simple Sequence Repeat) markers enabling rapid creation of a mapping population. This study successfully identified 12 SSR markers for the testing of Jaeger 70, Vignoles and their offspring for hybrid confirmation. Out of the 508 possible Jaeger 70 x Vignoles progeny, 346 plants (68.1%) were determined to be true hybrids. Hybrid confirmation using SSR saves time, money, labor and materials due to not needing to maintain plants until maturity (3 to 5 years). Developing a mapping population is an important first step in a breeding program.

Copyright

© Carl William Knuckles IV

Open Access

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