Bacteriophage Sp10 Infection of Bacillus Subtilis
Date of Graduation
Master of Science in Biology
Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells.
© Wayne Charles Katt
Katt, Wayne Charles, "Bacteriophage Sp10 Infection of Bacillus Subtilis" (1985). MSU Graduate Theses. 67.