Steroid Stimulated Protein Synthesis in Fish Cell Cultures
Date of Graduation
Master of Science in Biology
Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.
© Carla B Green
Green, Carla B., "Steroid Stimulated Protein Synthesis in Fish Cell Cultures" (1986). MSU Graduate Theses. 69.