Thesis Title

Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes

Date of Graduation

Summer 1979

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Richard Myers

Subject Categories

Biology

Abstract

This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell.

Copyright

© Laura Lynn Vanlandingham

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