Thesis Title

Reproduction and Movement Patterns of the Common Snapping Turtle (Chelydra Serpentina) Inhabiting a Hypothermic Reservoir in Southwest Missouri

Date of Graduation

Spring 1999

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Don Moll

Subject Categories

Biology

Abstract

Candida albicans is a frequent opportunistic pathogen of immunocompromised hosts. The switch from the yeast to the hyphal form enhances virulence. Murine peritoneal (PM⏀) and alveolar macrophages (AM⏀) produce a variety of cytokines as well as nitric oxide (NO), a short lived radical gas, in response to C. albicans. PM⏀ and AM⏀ were harvested and stimulated with C. albicans yeast or hyphae alone or a combination of interferon-gamma (IFN-⋎) plus C. albicans yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did not produce detectable levels until 48 hr. Neither PM⏀ or AM⏀ produced detectable levels of NO in response to C. albicans yeast or hyphae alone. In response to C. albicans yeast or hyphae plus IFN-⋎, PM⏀ produced NO at concentrations less than 1 uM. However, AM⏀ produced NO at concentrations approximating 15 uM in response to C. albicans yeast plus IFN-⋎and at concentrations approximating 45 uM in response to C. albicans hyphae plus IFN-⋎. This study indicates that PM⏀ and AM⏀ respond differently to challenge, possibly through different processing pathways.

Copyright

© Timothy B Wilson

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