Thesis Title

The Population and Activity of Airway Macrophages in Patients Requiring Continuous Mechanical Ventilation

Date of Graduation

Fall 1988

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Richard Myers

Subject Categories

Biology

Abstract

Macrophages are present in the trachea and bronchial airways of patients receiving continuous mechanical ventilation yet their functional relationship with other cell types and role as phagocytes is not well defined. In this study a technique utilizing a high speed blender was developed to allow separation of the adherent cell population from highly mucoid specimens. Many adherent cells failed to demonstrate phagocytosis. Those that did demonstrated above normal phagocytic ability when challenged with Aspergillus fumigatus conidia. The cellular content of the specimens demonstrated a predominance of polymorphonuclear neutrophils and a prevalence of various bacteria. These observations may indicate preactivated macrophages in an inflammatory environment perpetuated by the constant presence of bacteria and the mechanical insult of frequent trachael suctioning.

Copyright

© Thomas Dieckhoff

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Dissertation/Thesis

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