Date of Graduation

Spring 2016

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Chin-Feng Hwang

Keywords

grapes, leaf shape, morphology, genetics, genome-wide association study (GWAS), single-nucleotide polymorphism (SNP), genotyping-by-sequencing (GBS)

Subject Categories

Plant Sciences

Abstract

Leaves are a highly distinguishing characteristic in grape (Vitis vinifera) and display great diversity in comparison to other crops. However, little is known about the genetic basis of leaf shape in grape. Here, morphometrics and 40,724 single-nucleotide polymorphisms (SNPs) are correlated to describe the relationship between genotype and phenotype of leaf shape in grape. Parent vines (Norton and Cabernet Sauvignon) and their F1 progeny make up the mapping population located at the State Fruit Experiment Station, Genomics Research Vineyard on Missouri State University campus in Mountain Grove, Missouri. Important leaf shape characteristics (n=17) were identified based on the venation pattern, lobes, and sinuses. Morphometric analysis quantified overall leaf shape and SNP data were used to identify potential quantitative trait loci (QTL) responsible for leaf shape. A General Procrustes Analysis (GPA) produced trait measurements in the form of principal component (PC) scores. Generalized linear model (GLM) and composite interval mapping (CIM) analyses correlated trait measurements and SNPs data to identify three possible QTLs (located on chromosomes 1, 8, and 17) associated with leaf shape in this population. Post-hoc statistical analyses (Bonferroni correction and Benjamini-Hochberg adjustment) indicated SNPs on each of the three chromosomes with statistically significant association (p<0.08) to leaf shape in this population.

Copyright

© Brigette Rachelle Williams

Open Access

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