Thesis Title

Macrophage Suppression Across Genetic Barriers By a Spleen Cell Culture Derived Factor

Date of Graduation

Fall 1985

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Richard Myers

Subject Categories

Biology

Abstract

Supernatant from Concanavalin A stimulated murine spleen cell cultures has been shown to contain a factor which suppresses in vitro phagocytosis of Listeria monocytogenes by resident murine peritoneal macrophages (PEC). The current investigation involved production of this macrophage suppressor factor (MSF) by spleen cells obtained from C57BL/10J and BALB/c mice and an assay for the maintenance of biological activity across genetic barriers. Suppression of phagocytic activity of C57BL/10J PEC by MSF derived from BALB/c mice and likewise, activity of BALB/c PEC with MSF from C57BL/10J mice was compared to controls. The degree of suppression by heterologous MSF did not differ significantly from that in homologous systems. These results indicate that MSF produced by murine strains studies was not genetically restricted in its activity towards macrophages.

Copyright

© Kenneth L Mackey

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Dissertation/Thesis

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