Stress response proteins' differential expression in embryogenic and non-embryogenic callus of Vitis vinifera L. cv. Cabernet Sauvignon-A proteomic approach

Abstract

Co-cultivation of grape embryogenic callus (EC) and somatic embryos with Agrobacterium often leads to necrogenesis, which manifests in tissue browning and subsequent cell death. Interestingly, however, co-cultivation of grape non-embryogenic callus (NEC) does not display any visible symptoms. This paper compares the proteome of EC and NEC of Vitis vinifera L. cv. Cabernet Sauvignon to investigate the underlying mechanism. Analysis of 2-DE gels revealed 1180 spots, with 154 of them detected as differentially responsive proteins in EC versus NEC. Of these, the identity of 108 proteins was determined, with 28 proteins falling in the stress-response category. Those exclusively or predominantly identified in EC were iron-deficiency-responsive protein, acidic ascorbate peroxidase and isoflavone reductase-like protein, and those that were exclusively or predominant in NEC were basic ascorbate peroxidase, catalase, calcineurin B-like protein, 1,3-β-glucanase and cyclin-dependent kinase A1. The abundant protein PR-10 was at a remarkably lower level in EC than in NEC. These results demonstrated that different stress response pathways were activated in EC from those in NEC. A number of additional differentially expressed proteins involved in various functional categories were also identified.

Department(s)

Biology

Document Type

Article

DOI

https://doi.org/10.1016/j.plantsci.2009.04.003

Keywords

Embryogenic callus, MALDI-TOF-MS, Proteomics, Stress response proteins, Vitis vinifera L

Publication Date

8-1-2009

Journal Title

Plant Science

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