Abstract

Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan for a laboratory-based course with the goal of editing the genome of budding yeast, Saccharomyces cerevisiae. Students apply knowledge regarding advanced topics such as: CRISPR/Cas9 gene editing, DNA repair, genetics, and cloning. The lesson requires students to master skills such as bioinformatics analysis, restriction enzyme digestion, ligation, basic microbiology skills, polymerase chain reaction, and plasmid purification. Instructors are led through the technical aspects of the protocols, as well as the teaching philosophy involved throughout the laboratory experience. As it stands, the laboratory lesson is appropriate for 6-8 weeks of an upper-level undergraduate laboratory course, but may be adapted for shorter stints and students with less experience. Students complete the lesson with a more realistic idea of life science research and report significant learning gains. I anticipate this lesson to provide instructors and students in undergraduate programs with a hands-on, discovery-based learning experience that allows students to cultivate skills essential for success in the life sciences.

Description

This work was supported by the Biomedical Sciences Department at Missouri State University and first published by CourseSource.

Material consists of Lesson and six supporting documents.

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Download Full Text (853 KB)

Download S1: CRISPR in Yeast - Lecture PowerPoint (23.8 MB)

Download S2: CRISPR in Yeast - Sample Assessments (210 KB)

Download S3: CRISPR in Yeast - Laboratory Manual (1.1 MB)

Download S4: CRISPR in Yeast - Instructor Notes (38 KB)

Download S5: CRISPR in Yeast - Lab Report Rubric (22 KB)

Download S6: CRISPR in Yeast - Data Analysis (21 KB)

Creative Commons License

Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.

Publication Date

5-31-2019

Keywords

CRISPR/Cas9, gene editing, yeast, college life-science course, laboratory exercise

Disciplines

Biology | Fungi | Genetics

CRISPR/Cas9 in Yeast: A Multi-week Laboratory Exercise for Undergraduate Students

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