Mary M. Dean

Date of Graduation

Spring 2008


Master of Natural and Applied Science in Agriculture


College of Agriculture

Committee Chair

Gary Webb


stallion, spermatozoa, centrifuge, percent recovery, post-storage

Subject Categories



Two experiments were conducted in order to compare the effects of centrifugation on post-storage motility, percent intact acrosomes, detached heads and recovery of spermatozoa. In experiment 1, one aliquot was diluted with extender and placed in a 50 ml tube and centrifuged for 700 x g for 15 min. Additional aliquots were diluted 1:1, placed in 15 ml tubes and centrifuged at 400 x g for 7 min. In experiment 2, two aliquots were diluted and placed in 50 ml tubes and centrifuged for either 700 x g for 15 min or 600 x g for 12 min. The procedure for centrifugation at 400 x g was repeated. Following centrifugation, aliquots from each treatment were stored at 5o C or frozen in liquid nitrogen. In experiment 1, cold stored aliquots centrifuged for 400 x g yielded higher total and progressive motility, average path and straight line velocity and percent recovery for cold stored treatments (P≤ 0.05). In experiment 2, cold storage total motility was significantly higher (P≤ 0.01) for aliquots centrifuged at 400 x g in 15 ml tubes as compared to those centrifuged in 50 ml tubes at higher speeds and longer times. Post-thaw motility was not different between centrifugation treatments for either experiment. Following centrifugation, the percent recovery was higher (P≤ 0.01) for aliquots centrifuged in the 15 ml vs. 50 ml tubes for both experiments. However, spermatozoal integrity was not different between treatments for both experiments.


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