Date of Graduation

Summer 2020

Degree

Master of Science in Chemistry

Department

Chemistry

Committee Chair

Keiichi Yoshimatsu

Keywords

carbon nanoparticle, deoxyribonucleic acid detection, dot blotting, nitrocellulose membrane, fluorescent ink

Subject Categories

Analytical Chemistry | Biochemistry

Abstract

There is a significant interest in developing a sensitive, selective, efficient, and inexpensive method for rapid molecular diagnostic tests. This research aims to develop an inexpensive nucleic acid detection method by using DNA-conjugated carbon nanoparticles that exhibit fluorescence in the visible region. Carbon nanoparticles of this class can be detected without specialized equipment and have great promise toward the development of analytical methods that can be used in resource-limited environments with a lack of access to proper diagnostic and healthcare. We employed EDC-NHS (two-step) and EDC (one-step) coupling techniques to prepare DNA-conjugated carbon nanoparticles. The dot blotting method was adapted as a format in evaluating the applicability of the prepared nanoparticles in DNA detection. Along with the pursuit of nucleic acid detection method development, we found that the carbon nanoparticles bind to filter papers that are made of cellulose. By capitalizing on this property of carbon nanoparticles, the potential utility of carbon nanoparticles as a new fluorescent ink has also been investigated.

Copyright

© Luckio Frank Owuocha

Available for download on Monday, August 01, 2022

Open Access

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