Date of Graduation

Spring 2024


Master of Science in Plant Science (Agriculture)


College of Agriculture

Committee Chair

Chin-Feng Hwang


The susceptibility of grapevines to low-temperature injury presents a significant challenge, particularly in the United States. Traditional European grape cultivar, Vitis vinifera ‘Cabernet sauvignon’, faces bud damage at temperatures below freezing. On the other hand, Vitis interspecific hybrid ‘Chambourcin’, exhibits greater tolerance to sub-zero temperatures. The objective of this research was to conduct Differential Thermal Analysis (DTA) on 273 F1 hybrid genotypes generated from a cross of ‘Chambourcin’ × ‘Cabernet Sauvignon’ to quantify bud cold hardiness by identifying the freezing exothermic reactions. Low temperature exotherm (LTE) profiles, over two dormant seasons (December to February 2022-2024), revealed 15 interspecific hybrid cultivars with superior midwinter cold hardiness, where maximum levels of hardiness were observed between -25 °C to -28 °C. Notably, two promising genotypes, ChxCs012 and ChxCs279, exhibited the most consistent cold hardiness across two dormant seasons. Additionally, 355 simple sequence repeats (SSR) and 1,394 RNaseH2-dependent amplicon sequencing (rhAmpSeq)-derived haplotype markers were used to construct a linkage map which was used in downstream Quantitative Trait Loci (QTL) analysis for cold hardiness. Significant QTLs were discovered on linkage groups (LGs) 1, 9, and 11 that explained 13.86 % to 19.67 % of total phenotypic variance. These results can promote hybrid cultivar selection via cold hardy linked markers that can be applied in marker-assisted selection (MAS) breeding, to identify grapevine cultivars that can thrive under wider climatic conditions, benefiting the wine and grape industry.


grapevine cold hardiness, differential thermal analysis, hybrid grape genotypes, low-temperature exotherms, quantitative trait analysis, SSRs, rhAmpSeq, climate change, marker-assisted selection breeding

Subject Categories

Horticulture | Plant Breeding and Genetics | Viticulture and Oenology


© Tabinda Shahid

Available for download on Wednesday, January 01, 2025

Open Access