Date of Graduation
Spring 2024
Degree
Master of Science in Plant Science (Agriculture)
Department
College of Agriculture
Committee Chair
Chin-Feng Hwang
Abstract
The susceptibility of grapevines to low-temperature injury presents a significant challenge, particularly in the United States. Traditional European grape cultivar, Vitis vinifera ‘Cabernet sauvignon’, faces bud damage at temperatures below freezing. On the other hand, Vitis interspecific hybrid ‘Chambourcin’, exhibits greater tolerance to sub-zero temperatures. The objective of this research was to conduct Differential Thermal Analysis (DTA) on 273 F1 hybrid genotypes generated from a cross of ‘Chambourcin’ × ‘Cabernet Sauvignon’ to quantify bud cold hardiness by identifying the freezing exothermic reactions. Low temperature exotherm (LTE) profiles, over two dormant seasons (December to February 2022-2024), revealed 15 interspecific hybrid cultivars with superior midwinter cold hardiness, where maximum levels of hardiness were observed between -25 °C to -28 °C. Notably, two promising genotypes, ChxCs012 and ChxCs279, exhibited the most consistent cold hardiness across two dormant seasons. Additionally, 355 simple sequence repeats (SSR) and 1,394 RNaseH2-dependent amplicon sequencing (rhAmpSeq)-derived haplotype markers were used to construct a linkage map which was used in downstream Quantitative Trait Loci (QTL) analysis for cold hardiness. Significant QTLs were discovered on linkage groups (LGs) 1, 9, and 11 that explained 13.86 % to 19.67 % of total phenotypic variance. These results can promote hybrid cultivar selection via cold hardy linked markers that can be applied in marker-assisted selection (MAS) breeding, to identify grapevine cultivars that can thrive under wider climatic conditions, benefiting the wine and grape industry.
Keywords
grapevine cold hardiness, differential thermal analysis, hybrid grape genotypes, low-temperature exotherms, quantitative trait analysis, SSRs, rhAmpSeq, climate change, marker-assisted selection breeding
Subject Categories
Horticulture | Plant Breeding and Genetics | Viticulture and Oenology
Copyright
© Tabinda Shahid
Recommended Citation
Shahid, Tabinda, "Cold Hardiness in Vitis Interspecific Hybrid 'Chambourcin'-Based Population" (2024). MSU Graduate Theses. 3967.
https://bearworks.missouristate.edu/theses/3967
Open Access
Included in
Horticulture Commons, Plant Breeding and Genetics Commons, Viticulture and Oenology Commons
