Title
Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6
Abstract
Chromosome banding analysis of human malignant melanoma has documented the nonrandom alteration of chromosome 6. To determine the relevance of chromosome 6 abnormalities in melanoma, a normal chromosome 6 was directly introduced into melanoma cell lines. The resulting (+6) microcell hybrids were significantly altered in their phenotypic properties in culture and lost their ability to form tumors in nude mice. The loss of the chromosome 6 from melanoma microcell hybrids resulted in the reversion to tumorigenicity of these cells in mice. The introduction of the selectable marker (psv neo) alone into melanoma cell lines had no effect on tumorigenicity. These results support the idea that one or more genes on chromosome 6 may control the malignant expression of human melanoma.
Document Type
Article
DOI
https://doi.org/10.1126/science.2300817
Publication Date
1-1-1990
Recommended Citation
Trent, Jeffrey M., Eric J. Stanbridge, Heyoung L. McBride, Eckart U. Meese, Graham Casey, Diana E. Araujo, Colette M. Witkowski, and Raymond B. Nagle. "Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6." Science 247, no. 4942 (1990): 568-571.
Journal Title
Science
