Preparation of postimplantation mouse embryos for imaging

Abstract

Genetic manipulation methods allow fluorescent labeling of virtually any cell type or protein of interest in developing embryos, providing powerful insights into morphogenetic events at cellular and subcellular resolutions. The development of ex vivo embryo culture methods combined with high-resolution imaging provides a strong platform for observing morphogenetic events as they occur within the developing embryo. This protocol details the dissection and immobilization of postimplantation mouse embryos in preparation for live imaging.

Document Type

Article

DOI

https://doi.org/10.1101/pdb.prot5594

Publication Date

4-1-2011

Journal Title

Cold Spring Harbor Protocols

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