Facile Synthesis of a Fluorescent Deoxycytidine Analogue Suitable for Probing the RecA Nucleoprotein Filament
Abstract
We report the synthesis of the fluorescent 2‘-deoxycytidine analogue 5-methylpyrimidin-2-one nucleoside, its incorporation at three specified sites in a single 60-nucleotide DNA molecule, and the use of its total and polarized intrinsic fluorescence to characterize RecA−DNA complexes.
Document Type
Article
DOI
https://doi.org/10.1021/ol0167863
Keywords
filaments, nucleic acids, genetics, monomers, fluorescence
Publication Date
2001
Recommended Citation
Singleton, Scott F., Feng Shan, Matthew W. Kanan, Catherine M. McIntosh, Chad J. Stearman, Jeremiah S. Helm, and Kristofor J. Webb. "Facile synthesis of a fluorescent deoxycytidine analogue suitable for probing the RecA nucleoprotein filament." Organic letters 3, no. 24 (2001): 3919-3922.
Journal Title
Organic letters
COinS
