Title
Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine
Abstract
Non-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvignon, which were referred to as VaNPR1.1 and VvNPR1. 1-CS, respectively. They encode a protein of 584 amino acids with a predicted molecular weight of 64.8 kDa and a theoretical isoelectric point (PI) of 5.74. The predicted amino acid sequences of VaNPR1.1 and VvNPR1.1-CS differ by only one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the transcriptional expression of AtPR-1 gene, though not to the full scale. This result demonstrated that a grapevine VaNPR1.1 possesses a similar function to the Arabidopsis NPR1 in the regulation of defense-related genes. Over-expression of VaNPR1.1 in transgenic Arabidopsis plant increased tolerance to salinity, but had no effect on the drought tolerance. We conclude that VaNPR1.1 is a functional ortholog of AtNPR1 and also involved in grapevine's response to the salt stress.
Department(s)
Environmental Plant Science and Natural Resources
Document Type
Article
DOI
https://doi.org/10.1016/S2095-3119(13)60432-3
Keywords
NPR1, Over-expression, Pathogenesis-related genee, Transgenic Arabidopsis
Publication Date
7-1-2013
Recommended Citation
Zhang, Yi-ming, Xi-lu NI, and Wenping Qiu. "Characterization of NPR1 genes from Norton and Cabernet sauvignon grapevine." Journal of Integrative Agriculture 12, no. 7 (2013): 1152-1161.
Journal Title
Journal of Integrative Agriculture
