Title
Rme1 is necessary for Mi-1-mediated resistance and acts early in the resistance pathway
Abstract
The tomato gene Mi-1 confers resistance to root-knot nematodes (Meloidogyne spp.), potato aphid, and whitefly. Using genetic screens, we have isolated a mutant, rme1 (resistance to Meloidogyne spp.), compromised in resistance to M. javanica and potato aphid. Here, we show that the rme1 mutant is also compromised in resistance to M. incognita, M. arenaria, and whitefly. In addition, using an Agrobacterium-mediated transient assay in leaves to express constitutive gain-of-function mutant Pto , we demonstrated that the rme1 mutation is not compromised in Pto-mediated hypersensitive response. Moreover, the mutation in rme1 does not result in increased virulence of pathogenic Pseudomonas syringae or Mi-1-virulent M. incognita. Using a chimeric Mi-1 construct, Mi-DS4, which confers constitutive cell death phenotype and A. rhizogenes root transformation, we showed that the Mi-1-mediated cell death pathway is intact in this mutant. Our results indicate that Rme1 is required for Mi-1-mediated resistance and acts either at the same step in the signal transduction pathway as Mi-1 or upstream of Mi-1. L205D
Document Type
Article
DOI
https://doi.org/10.1094/mpmi.2004.17.1.55
Publication Date
1-1-2004
Recommended Citation
de Ilarduya, Oscar Martinez, Gloria Nombela, Chin-Feng Hwang, Valerie M. Williamson, Mariano Muñiz, and Isgouhi Kaloshian. "Rme1 is necessary for Mi-1-mediated resistance and acts early in the resistance pathway." Molecular Plant-Microbe Interactions 17, no. 1 (2004): 55-61.
Journal Title
Molecular Plant-Microbe Interactions
