Title
Sperm concentration influences recovery of progressively motile spermatozoa and number of inseminations shipped in conventional containers
Abstract
Three ejaculates from each of 4 stallions were split into aliquots and diluted with skim milk-glucose extender to final concentrations of 25, 50, 100, 150, or 200 million progressively motile spermatozoa/ml. One aliquot per dilution was evaluated for the percentage progressive motility (PMS) and rate of forward movement (RFM) after 15 and 45 minutes of culture at 37°C. One aliquot was stored at 5°C for 24 hours and another for 48 hours at 5°C. The aliquots stored at 5°C were evaluated for PMS and RFM at 15 and 45 minutes of warming (37°C). For spermatozoa stored at 37°C following collection, concentration did not (P<.05) influence PMS or RFM. However, when spermatozoa were stored at 5°C for 24 or 48 hours, PMS and RFM declined (P<.05) as spermatozoal concentration increased. A concentration of 25 x 10 progressively motile spermatozoa/ml provided higher (P<.05) percentages of PMS following storage than did concentrations of 100,150, and 200 x 10M progressively motile spermatozoa/ml. However, in order to minimize insemination volume and maximize the number of inseminations available per shipment, a concentration of 100 x 10 progressively motile spermatozoa may be more appropriate than lower concentrations. © 1993, William E. Jones. All rights reserved. 6 6 6
Document Type
Article
DOI
https://doi.org/10.1016/S0737-0806(07)80260-7
Publication Date
1-1-1993
Recommended Citation
Webb, G. W., M. J. Ams, and K. C. Pool. "Sperm concentration influences recovery of progressively motile spermatozoa and number of inseminations shipped in conventional containers." Journal of Equine Veterinary Science 13, no. 9 (1993): 486-489.
Journal Title
Journal of Equine Veterinary Science