Sperm concentration influences recovery of progressively motile spermatozoa and number of inseminations shipped in conventional containers


Three ejaculates from each of 4 stallions were split into aliquots and diluted with skim milk-glucose extender to final concentrations of 25, 50, 100, 150, or 200 million progressively motile spermatozoa/ml. One aliquot per dilution was evaluated for the percentage progressive motility (PMS) and rate of forward movement (RFM) after 15 and 45 minutes of culture at 37°C. One aliquot was stored at 5°C for 24 hours and another for 48 hours at 5°C. The aliquots stored at 5°C were evaluated for PMS and RFM at 15 and 45 minutes of warming (37°C). For spermatozoa stored at 37°C following collection, concentration did not (P<.05) influence PMS or RFM. However, when spermatozoa were stored at 5°C for 24 or 48 hours, PMS and RFM declined (P<.05) as spermatozoal concentration increased. A concentration of 25 x 10 progressively motile spermatozoa/ml provided higher (P<.05) percentages of PMS following storage than did concentrations of 100,150, and 200 x 10M progressively motile spermatozoa/ml. However, in order to minimize insemination volume and maximize the number of inseminations available per shipment, a concentration of 100 x 10 progressively motile spermatozoa may be more appropriate than lower concentrations. © 1993, William E. Jones. All rights reserved. 6 6 6

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Journal of Equine Veterinary Science