The Little Sac Watershed in Greene and Polk Counties of southwest Missouri was placed on the 303d list for bacteria impairment in 1998 (WCO 2016). In 2006, a Total Maximum Daily Load (TMDL) was developed for the watershed to address bacteria impairments within the Little Sac River and an initial watershed management plan was finalized in 2010 (Baffaut 2006, WCO 2009). The Watershed Committee of the Ozarks (WCO) is presently updating that plan with the most recent information on bacteria within the watershed. As part of that process, the WCO has contracted the Ozarks Environmental and Water Resources Institute (OEWRI) at Missouri State University (MSU) to complete a bacteria source tracking study within the watershed to identify potential bacteria pollution sources. The purpose of this study is to collect water samples throughout the watershed and evaluate bacteria DNA using real-time PCR for specific marker genes that can help identify specific bacteria sources from different locations in the Little Sac River watershed.

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Completed for Watershed Committee of the Ozarks

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