Date of Graduation
Summer 2010
Degree
Master of Science in Chemistry
Department
Chemistry and Biochemistry
Committee Chair
Erich Steinle
Abstract
This thesis describes rapid, facile, selective, and specific solution and surface-based assays for the detection of proteins. Specifically, thrombin is used in both as a model system in conjunction with aptamer-gold nanoconjugates. The solution-based method utilizes Dynamic Light Scattering Spectroscopy (DLSS) as a method to detect increases in hydrodynamic diameter size of the conjugates. In the surface-based method, lateral flow technology is applied in conjunction with the aptamer-gold nanoconjugates to show both positive and control lines on the novel bound glass test strip. Both of these assays have significant biomedical and chemical applications.
Keywords
aptamer, gold nanoparticles, dynamic light scattering, lateral flow, bound glass, nanoconjugates, thrombin
Subject Categories
Chemistry
Copyright
© Tiffany Leah Severs
Recommended Citation
Severs, Tiffany Leah, "Synthesis, Modification, Characterization and Bioanalytical Applications of Aptamer-Gold Nanoconjugates" (2010). MSU Graduate Theses/Dissertations. 1884.
https://bearworks.missouristate.edu/theses/1884
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