Date of Graduation

Summer 2023

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Laszlo G. Kovacs

Abstract

The North American grapes species Vitis rupestris Scheele and Vitis riparia Michx have been the pillars of rootstock breeding for many decades. Though a large body of viticultural knowledge has been accumulated on their impact on grafted scions, the genetic basis of their root system architecture (RSA) has received limited scientific attention. In this study, I generated and analyzed adventitious root systems from dormant cuttings of 22 V. riparia and 19 V. rupestris accessions, as well as 162 interspecific F1 hybrid progeny from a cross between V. rupestris (♀) and V. riparia (♂). I photographed the roots and then extracted 23 traits of the RSA from 2-D images using the software RhizoVision Explorer. Principal component analysis (PCA) of seven uncorrelated root traits of the V. riparia and V. rupestris accessions showed that PC1 explained 57% of the phenotypic variance and arranged the two species into partially overlapping but clearly separate clusters. T-test results demonstrated greater mean for width (p = 0.00005), depth (p = 0.002), perimeter (p = 0.002), lower root area (p = 0.005), number of roots (p = 0.0001), and total root length (p = 0.002) in V. riparia, indicating that the overall size of the root system in this species tends to be greater than that in V. rupestris. Using a genotype-by-sequencing (GBS) marker-based integrated linkage map and a V. rupestris X V. riparia F1 progeny, I performed quantitative trait locus (QTL) analysis on 23 root traits. All significant loci were mapped to chromosome 10. A maternal QTL, which mapped to marker S10_4125692 at 21.1 cM, influenced several traits at pp

Keywords

root system architecture, root traits, 2-D images, Vitis rupestris, Vitis riparia, rootstock, QTL, linkage map, F1 hybrid progeny

Subject Categories

Agronomy and Crop Sciences | Botany | Molecular Genetics | Plant Biology | Plant Breeding and Genetics | Viticulture and Oenology

Copyright

© Parinaz Mohtasebi

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