Developing Onsite Detection Methods for Grapevine Vein Clearing Virus and Grapevine Red Blotch Virus
Date of Graduation
Winter 2023
Degree
Master of Science in Plant Science (Agriculture)
Department
College of Agriculture
Committee Chair
Wenping Qiu
Abstract
Both grapevine vein clearing virus and grapevine red blotch virus are detrimental pathogens on grapevines in the Midwestern United States. The most effective method of control for these viruses is early detection and removal of infected vines. A rapid and equipment-free method of detecting grapevine vein clearing virus and grapevine red blotch virus was developed using the isothermal DNA amplification technique, recombinase polymerase amplification, in conjunction with lateral flow strips. The method yields testing results under 35 minutes and can be performed onsite, and thus provides a rapid and grower-friendly diagnostic tool for preventing diseases caused by the two viruses.
Keywords
recombinase polymerase amplification, grapevine vein clearing virus, grapevine red blotch virus, lateral flow, onsite testing, rapid diagnostic
Copyright
© Gregory Bryce Goodlett
Recommended Citation
Goodlett, Gregory Bryce, "Developing Onsite Detection Methods for Grapevine Vein Clearing Virus and Grapevine Red Blotch Virus" (2023). MSU Graduate Theses/Dissertations. 3932.
https://bearworks.missouristate.edu/theses/3932
Open Access
