Date of Graduation

Spring 2024

Degree

Master of Science in Biology

Department

Biology

Committee Chair

Laszlo G. Kovacs

Abstract

In grapevine, the influence of the plant’s elemental composition (the ionome) on fruit and wine quality is well established, but its genetic underpinnings have received limited scientific attention. In this study, I analyzed the leaf ionome of 131 interspecific F1 hybrid progeny plants from a cross between a Vitis rupestris Scheele (♀) and a Vitis riparia Michx (♂) parents, which were replicated in four different environments: Southwest Missouri, Central Missouri, South Dakota, and New York. I sampled leaves at three different times during the growing season at all four locations and had the concentration of 20 elements measured using ICP-MS. Positive and negative correlations among the concentration of various elements were consistent across the four geographic locations in the highest-node leaves at anthesis. Correlation in other leaf samples were limited mostly between the South Dakota, and New York locations. Varimax-rotated Principal component analysis (PCA) performed on the leaf ionome demonstrated that PC1 and PC2 accounted for 17.30% and 10.50% of variance, respectively. Using a GBS-based linkage map and the ionomic profile as a phenotype, 9 recurring QTL were mapped, indicating the presence of genomic regions which influence the accumulation of individual elements. Three QTL were identified at p0.0125. Interestingly, this PCA-derived QTL mapped to the same marker as the QTL peak for K concentration. The novel leaf ionome QTL identified in this study further our understanding of the genetic basis of elemental composition in grapevine and offer actionable data for markerassisted selection

Keywords

ionome, QTL, Vitis rupestris, Vitis riparia, rootstock, linkage map, F1 hybrids

Subject Categories

Genetics | Molecular Genetics | Plant Breeding and Genetics

Copyright

© Jesse L. Krokower

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Open Access

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