Date of Graduation

Spring 2025

Degree

Master of Science in Plant Science (Agriculture)

Department

College of Agriculture

Committee Chair

Chin-Feng Hwang

Abstract

Berry juice color is one of the key factors influencing grape and wine quality, consumer preference, and marketability. While most previous studies on berry color have focused on the skin, research on juice color remains limited. This study investigated the genetic basis of berry juice color in a hybrid population derived from the Vitis interspecific hybrid ‘Chambourcin’ and V. vinifera ‘Cabernet Sauvignon’. A new nine-category visual scoring system was developed to assess juice color based on OIV 225. To achieve comprehensive precision and quantitative analysis, CIELAB parameters (L*, a*, b*), Hue, and Chroma were extracted from image-based measurements. Using berries harvested over four different years, a stable QTL associated with juice color was identified on linkage group (LG) 2 within a 4.8 cM interval. Two peak markers: rh_chr2_14239122 and rh_chr2_14464718, were closely linked to the QTL, which co-localizes with three MYBA genes involved in anthocyanin synthesis. The haplotypes associated with the pigmentation alleles at these two rhAmpSeq markers can be used to identify Noir varieties with an accuracy of 97.2%. These findings provide a valuable resource for marker-assisted selection in grape breeding programs to develop cultivars with specific juice color traits while also contributing to a broader understanding of the genetic regulation of anthocyanin-related traits.

Keywords

QTL mapping, Grapevine juice color, Grapevine breeding, marker-assisted selection, rhAmpSeq marker, SSR marker

Subject Categories

Plant Breeding and Genetics | Viticulture and Oenology

Copyright

© Qiuni Yang

Available for download on Wednesday, December 31, 2025

Open Access

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